NAD+

NAD+ (nicotinamide adenine dinucleotide) is a dinucleotide coenzyme (not a peptide, CAS 53-84-9) made up of two nucleotides – adenine and nicotinamide. It is one of the central redox carriers: in laboratory models it acts as an electron carrier, switching between its oxidised (NAD+) and reduced (NADH) forms. In the research context it is also assessed as a substrate and cofactor of NAD+-dependent enzymes – sirtuins (SIRT) and poly(ADP-ribose) polymerases (PARP). This information is intended for in vitro research only and is not an instruction for use.

In in vitro models, NAD+ is used to study the redox reactions of cellular energy metabolism – as an electron carrier in experiments on glycolysis, the citric acid (Krebs) cycle and oxidative phosphorylation. Cell cultures are used to analyse how the NAD+/NADH ratio affects redox balance, mitochondrial function and DNA repair signalling pathways. A separate area of research is its role as a cofactor of sirtuins (e.g. the SIRT1 deacetylase) and PARP enzymes; peer-reviewed in vitro work describes the enzymatic activity of sirtuins as dependent on NAD+ availability. All data apply only in the context of laboratory research.

The product is supplied as a lyophilised powder of ≥99% purity (HPLC). Each vial contains 1000 mg of NAD+. For laboratory handling, the powder is usually dissolved in bacteriostatic water (sold separately), poured slowly along the side of the vial; the vial is swirled gently in a circle until the powder has fully dissolved – it is not shaken.

Keep the unopened vial of dry powder in a refrigerator (+2…+8 °C), or in a freezer (−20 °C) for longer storage, protected from light and moisture. Once reconstituted in bacteriostatic water, keep the solution refrigerated and use it within the time set out in your research protocol.

In in vitro research, NAD+ is most often studied together with the enzyme systems that depend on it – sirtuins (SIRT1–SIRT7) and PARP enzymes – as their substrate and cofactor. In redox experiments it is assessed as a pair with its reduced form NADH, analysing the NAD+/NADH ratio. Typical models include cell culture studies of mitochondrial function and DNA repair signalling pathways. No dosing instructions are given – the information is intended for the laboratory context only.

Absolutely not. This product is sold exclusively for laboratory research and scientific experiments. It is not intended for human or animal consumption and is not a medicine, dietary supplement or cosmetic product.